Cicer arietinum primers V2 [Varshney et al.]

Intron-spanning region (ISR) markers based on regions conserved with chickpea (Cicer arietinum)

Method:

Chickpea transcript assembly contigs (CaTA v2) were aligned with the Medicago genome using Exonerate. Primer pairs were selected across intron boundaries using BatchPrimer3 and custom scripts. See reference below for details.

References:

  1. Comprehensive transcriptome assembly of chickpea (Cicer arietinum) using Sanger and Next Generation Sequencing platforms: Development and applications. Kudapa ... Varshney et al., submitted